Cloning and characterisation of NMDA receptors in the Pacific oyster, Crassostrea gigas (Thunberg, 1793) in relation to metamorphosis and catecholamine synthesis

نویسندگان

چکیده

Bivalve metamorphosis is a developmental transition from free-living larva to benthic juvenile (spat), regulated by complex interaction of neurotransmitters and neurohormones such as L-DOPA epinephrine (catecholamine). We recently suggested an N-Methyl-D-aspartate (NMDA) receptor pathway additional previously unknown regulator bivalve metamorphosis. To explore this theory further, we successfully induced in the Pacific oyster, Crassostrea gigas, exposing competent larvae L-DOPA, epinephrine, MK-801 ifenprodil. Subsequently, cloned three NMDA subunits CgNR1, CgNR2A CgNR2B, with sequence analysis suggesting successful assembly functional complexes binding natural occurring agonists channel blocker MK-801. are expressed larvae, during spat, but expression neither self-regulated nor catecholamines. In-situ hybridisation CgNR1 identified presence apical organ/cerebral ganglia area potential sensory function, nervous network foot indicating putative muscle regulatory function. Furthermore, phylogenetic analyses molluscan-specific gene expansions key enzymes involved catecholamine biosynthesis. However, exposure did not alter selected enzymes, that receptors do regulate biosynthesis catecholamines via expression.

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ژورنال

عنوان ژورنال: Developmental Biology

سال: 2021

ISSN: ['0012-1606', '1095-564X']

DOI: https://doi.org/10.1016/j.ydbio.2020.10.008